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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Nanoparticle labeling identifies slow cycling human endometrial stromal cells

Figure 4

The cell proliferation activity (A - E) and G 1 -phase length (F - H) of human endometrial stromal FPC and non-FPC. Representative photographs of (A) unselected stromal cells, (B) FPC and (C) non-FPC morphology in the proliferation assay at day 5. Cell proliferation assessment on unselected endometrial stromal cells (grey), clonally derived FPC (white) and non-FPC (black) after 15 days in culture using (D) trypan blue assay and (E) CyQUANT NF cell proliferation assay. The absorbance unit (AU) was measured at a wavelength of 480 nm with a reference wavelength of 530 nm. Scale bar: 100 μM. Cells were obtained from the same patient. Results are reported as means ± SEM, n = 3, *P <0.05. Quantification of the G1 length in Cdt-1-expressing stromal cells of FPC and non-FPC populations followed by time-lapse microscopy within a period of 42 hours. The distribution of G1-phase for (F) unselected stromal cells (n = 20 cells), (G) FPC (n = 20 cells) and (H) non-FPC (n = 18 cells) from three patient samples. FPC, fluorescent persistent cells; SEM, standard error of the mean.

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