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Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Tendon proper- and peritenon-derived progenitor cells have unique tenogenic properties

Figure 6

Fibrillar collagens were present in the tendon constructs. Staining for collagens I, III and V was analyzed in P1 Achilles tendon and progenitor-derived tendon constructs at Day 7. Because the tissue and cells originate from ScxGFP transgenic mice, cells with an active Scx promoter contain green fluorescent protein (GFP). In the P1 Achilles tendon, GFP allows for delineation (white dashed line) of peritenon (peri) and tendon proper (tp) (A-D). Active GFP was also detected for TP-derived progenitors in constructs (white arrows, E-L). As with the P1 Achilles tendon, TP, TP + GDF5, PERI, and PERI + GDF5 constructs were positive for staining for collagen I (A, E, I, M, Q), collagen III (B, F, J, N, R) and collagen V (C, G, K, O, S). Negative controls represent staining with secondary antibody only (D, H, L, P, T) (Bar: 100 μm).

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