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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

Figure 4

Tri-lineage differentiation. A-I) Representative images of XF,SFM-cultured and DMEM KO + 10% FBS-cultured MSCs, differentiated into adipocytes, osteocytes and chondrocytes, as visualized by Oil Red O, von Kossa and alcian blue stains, respectively, against undifferentiated controls; J) Bar graph to quantify oil droplets, post Oil Red O staining; K) Bar graph to quantify mineralization, post alizarin red staining; L) Bar graph to quantify sulfated glycosaminoglycan production by differentiated chondrocytes, normalized to DNA content. FBS, fetal bovine serum; MSCs, mesenchymal stem cells.

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