Figure 5

Immunomodulation, karyotype and cell cycle analysis. A) Bar graph showing percentage suppression of PBMC-generated MLRs, by cells cultured in XF,SF medium, against those cultured in DMEM KO + 10% FBS, at passages 4 and 6; B) Percentage proliferation brought about by co-culturing MSCs (cultured in both conditions) with human peripheral blood-derived PBMCs, at passages 4 and 6, shown against a one-way mixed lymphocyte reaction taken as 100% proliferation; C and D) Representative karyotypes of cells cultured in XF,SF medium and DMEM KO + 10% FBS, respectively, at passage 5; E) Percentage of cells cultured in XF,SFM and DMEM KO + 10% FBS, in S phase at passage 5; F) Percentage of cells cultured in XF,SF medium and DMEM KO + 10% FBS in G0/G1, S and M phases, at passage 5. FBS, fetal bovine serum; PBMC, peripheral blood mononuclear cells.