Figure 6

Angiogenic potency. A, B) Real-time PCR quantification of HGF, VEGF, TGFβ-1 and IL6 for cells cultured in DMEM KO + 10% FBS and in XF,SFM, all normalized against Cord 1 (DMEM KO + 10% FBS); C,D,E) Box graphs representing variations observed between cells cultured in XF,SFM and FBS-containing medium, when analyzed for cytokine secretions of TGFβ, HGF and Ang1; F) Tube formation efficiency (estimated using HUVEC, cultured on growth factor reduced Matrigel) of WJ-MSC-conditioned medium, from three cords cultured in DMEM KO + 10% FBS and in XF, SFM. Anti-HGF neutralizing antibody was used to block tube formation in each condition. G-J’) Representative 4 X pictures showing tubes formed. FBS, fetal bovine serum; HGF, hepatocyte growth factor; TGFβ1, transforming growth factor-β1; VEGF, vascular endothelial growth factor. ** signifies extent of significance of the numeric value.