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Figure 7 | Stem Cell Research & Therapy

Figure 7

From: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

Figure 7

Senescence, apoptosis and transformation. A) Percentage of senescent cells in XF,SFM cultures, compared against DMEM KO + 10% FBS cultures at P5 followed by representative 10 X images of fields showing blue colored senescent cells; B) Percentage of Annexin positive (apoptotic) cells in XF,SFM cultures compared to DMEM KO + 10% FBS cultures at P5 followed by representative images of fields showing green colored apoptotic cells; C) Representative pictures of cells cultured in soft agar medium, showing no colonies in XF,SFM and DMEM KO + 10% FBS cultures, compared to colonies observed in MCF-7 cultures; D) Comparative gene expression analysis between cells cultured in XF,SF medium and DMEM KO + 10% FBS at P6. FBS, fetal bovine serum; P, passage.

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