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Table 1 Antibodies used for flow cytometry analysis

From: Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

Antibody Fluorochrome Catalogue number Stock concentraton Antibody dilution Isotype dilution
CD73 PE 550257, BD 12.5 µg/ml NEAT 1:16 ISO PE
CD44 PE 550989, BD 12.5 µg/ml NEAT 1:16 ISO PE
CD166 PE 559263, BD 12.5 µg/ml NEAT 1:16 ISO PE
CD90 PE 555596, BD 0.2 mg/ml NEAT NEAT ISO PE
CD105 FITC FAB10971F, R&D 50 µg/ml NEAT 1:10 ISO FITC
CD200 PE 552475, BD 0.25 µg/20 ml NEAT NEAT ISO PE
HLA ABC APC 555555, BD 3 µg/ml NEAT 1:4 ISO APC
CD34 PE 550761, BD 12.5 µg/ml NEAT 1:16 ISO PE
CD271 PE 557196, BD 0.125 µg/20 ml NEAT NEAT ISO PE
CD80 PE 340294, BD 0.0032 mg/ml NEAT 1:62.5 ISO PE
CD45 FITC 555482, BD 6.25 µg/ml NEAT 1:80 ISO FITC
HLA DR FITC 347663, BD 25 µg/ml NEAT 1:2 ISO FITC
  1. APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin.