Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study

Table 1 Antibodies used for flow cytometry analysis

Antibody	Fluorochrome	Catalogue number	Stock concentraton	Antibody dilution	Isotype dilution
CD73	PE	550257, BD	12.5 µg/ml	NEAT	1:16 ISO PE
CD44	PE	550989, BD	12.5 µg/ml	NEAT	1:16 ISO PE
CD166	PE	559263, BD	12.5 µg/ml	NEAT	1:16 ISO PE
CD90	PE	555596, BD	0.2 mg/ml	NEAT	NEAT ISO PE
CD105	FITC	FAB10971F, R&D	50 µg/ml	NEAT	1:10 ISO FITC
CD200	PE	552475, BD	0.25 µg/20 ml	NEAT	NEAT ISO PE
HLA ABC	APC	555555, BD	3 µg/ml	NEAT	1:4 ISO APC
CD34	PE	550761, BD	12.5 µg/ml	NEAT	1:16 ISO PE
CD271	PE	557196, BD	0.125 µg/20 ml	NEAT	NEAT ISO PE
CD80	PE	340294, BD	0.0032 mg/ml	NEAT	1:62.5 ISO PE
CD45	FITC	555482, BD	6.25 µg/ml	NEAT	1:80 ISO FITC
HLA DR	FITC	347663, BD	25 µg/ml	NEAT	1:2 ISO FITC

ISSN: 1757-6512