Table 2 List of primers used for RT-PCR analysis
Markers | Forward primer | Reverse primer | Tm | Product length |
|---|---|---|---|---|
β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT | 60 | 250 bp |
P21 | GAGGCCGGGATGAGTTGGGAGGAG | CAGCCGGCGTTTGGAGTGGTAGAA | 63 | 220 bp |
P53 | TAACAGTTCCTGCATGGGCGGC | AGGACAGGCACAAACACGCACC | 60 | 121 bp |
P16 | TTATTTGAGCTTTGGTTCTG | CCGGCTTTCGTAGTTTTCAT | 51 | 354 bp |
XRCC4 | AAGATGTCTCATTCAGACTTG | CCGCTTATAAAGATCAGTCTC | 54 | 233 bp |
PTN | GGAGCTGAGTGCAAGCAAAC | GAGGTTTGGGCTTGGTCAGT | 59 | 274 bp |
MCM3 | TCGAGGTAACCTTTGGTGGC | TGTTCAGAAGCCTCGTCGTC | 55 | 232 bp |
CDKN2B | CCCAACTCCACCAGATAGCA | GGGATTTCCGCATCCTAGCA | 57 | 198 bp |
ERCC3 | CCAGGAAGCGGCACTATGAGG | GGTCGTCCTTCAGCGGCATTT | 61 | 171 bp |
Cmyc | AAGACTCCAGCGCCTTCTCTC | GTTTTCCAACTCCGGGATCTG | 62 | 526 bp |
VEGF-A | GCAGAATCATCACGAAGTGG | GCATGGTGATGTTGGACTCC | 60 | 234 bp |
TGF-β | CAGATCCTGTCCAAGCTG | TCGGAGCTCTGATGGTGTT | 56 | 270 bp |
Ang-1 | GCTTACCAGATTCACACTGTTCC | TTGCTACCTTGCCAACAACAACTG | 60 | 612 bp |
IL-6 | CACAGACAGCCACTCACCTC | TTTTCTGCCAGTGCCTCTTT | 60 | 137 bp |
HGF | ATGCATCCAAGGTCAAGGAG | TTCCATGTTCTTGTCCCACA | 61 | 349 bp |