Table 1 Primers used for RT-PCR[38]
From: Equine mesenchymal stromal cells and embryo-derived stem cells are immune privileged in vitro
Primer | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
18S | ATGCGGCGGCGTTATTCC | GCTATCAATCTGTCAACTCCT |
TNF-α | AAAGGACATCATGAGCACTGAAAG | GGGCCCCCTGCCTTCT |
IFN-γ | CTACCTATTACTGCCAGGCCG | TCCAGGAAAAGAGGCCCAC |
IL-6 | TGCTGGCTAAGCTGCATTCA | GGAAATCCTCAAGGCTTCGAA |
IL-8 | TTGGCCGTCTTCCTGCTTT | GGTTTGGAGTGCGTCTTGATG |
IP-10 | CCTCCAGTTGCAGCACCAT | TTCCTTGAGTTCCACTCAGAGTCA |
CCL5 | CACTGCCACCTTCTGCACTC | CGGGAGATGTAGGCAAAGCA |