Figure 2

Trilineage differentiation of head and neck squamous cell carcinoma-derived mesenchymal stromal cells. Tumor-derived mesenchymal stromal cells were harvested and subjected to trilineage differentiation using standard differentiation-inducing culture conditions. Top row: adipogenic differentiation after 14 days, staining performed with Sudan III. Middle row: chondrogenic differentiation after 14 days, staining with Alcian blue. Bottom row: osteogenic differentiation after 21 days, staining with Alizarin red. Images from one representative donor are shown.