Figure 7

Proposed schematic for miR-671-5p in orbital fat-derived stem cells regulating macrophage-mediated inflammation. (A) Under normal conditions, soluble tumor necrosis factor receptor type II (sTNF RII) and interleukin-1 receptor antagonist (IL-1 RA) in orbital fat-derived stem cells (OFSCs) are negatively regulated by miR-671-5p, while lipopolysaccharide (LPS) stimulation promotes tumor necrosis factor alpha (TNFα), interleukin (IL)-1α and IL-1β expression in macrophages. (B) Upon noncontact culture with LPS-activated macrophages, IL-10 and indoleamine 2,3 dioxygenase (IDO) in OFSCs are upregulated, while sTNFRII and IL-1 RA are rapidly produced by degradation of miR-671-5p in OFSCs. (C) Abundant sTNF RII and IL-1 RA in OFSCs neutralizes the proinflammatory effect from TNFα, IL-1α and IL-1β. iNOS, inducible nitric oxide synthase; TLR4, toll-like receptor 4.