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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Influence of insulin-like growth factor I overexpression via recombinant adeno-associated vector gene transfer upon the biological activities and differentiation potential of human bone marrow-derived mesenchymal stem cells

Figure 2

Recombinant adeno-associated virus-mediated IGF-I gene transfer in chondrogenically induced cultures of human bone marrow-derived mesenchymal stem cells. Human bone marrow-derived mesenchymal stem cell aggregates were transduced with rAAV-hIGF-I or rAAV-lacZ (40 μl each vector) as described in Methods and were histologically processed after 21 days to monitor (A) transgene (insulin-like growth factor I) expression by immunohistochemical analysis (magnification × 20) and enzyme-linked immunosorbent assay, and the levels of cell proliferation by immunohistochemical detection of bromodeoxyuridine (BrdU) incorporation and histomorphometry (B) (magnification × 10), by hematoxylin and eosin staining and histomorphometry (C) (magnification × 4), or by biochemical assays (WST-1 assay and detection of the DNA contents) (D). *Statistically significant compared with rAAV-lacZ. hIGF-I, human insulin-like growth factor I; OD, optical density; rAAV, recombinant adeno-associated virus.

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Stem Cell Research & Therapy

ISSN: 1757-6512