Skip to main content
Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Influence of insulin-like growth factor I overexpression via recombinant adeno-associated vector gene transfer upon the biological activities and differentiation potential of human bone marrow-derived mesenchymal stem cells

Figure 3

Metabolic and differentiation activities in chondrogenically induced cultures of human bone marrow-derived mesenchymal stem cells transduced with rAAV-hIGF-I. Human bone marrow-derived mesenchymal stem cell aggregates were transduced with rAAV-hIGF-I or rAAV-lacZ as described in Figure 2 and histologically processed after 21 days to evaluate the production of matrix proteoglycans (toluidine blue staining with histomorphometry and detection of the proteoglycan contents) (A) and the expression of SOX9 with histomorphometry (B) and type II collagen (specific immunodetection with histomorphometry and detection of the type II collagen contents) (C) (all at magnification × 4). *Statistically significant compared with rAAV-lacZ. hIGF-I, human insulin-like growth factor I; rAAV, recombinant adeno-associated virus.

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512