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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: TNFα and IL-1β influence the differentiation and migration of murine MSCs independently of the NF-κB pathway

Figure 1

Overexpression and functional activity of super-repressor inhibitor of nuclear factor-kappa-B (srIκB) in BALB/c mesenchymal stem cells (MSCs). Western blotting demonstrated that BALB/c MSCs transduced with AdsrIκB at multiplicity of infection (MOI) 1,000 expressed higher levels of IκB than cells transduced with adnull virus (A). MSCs were transduced with nuclear factor-kappa-B (NF-κB)-driven luciferase and either AdsrIκB or AdGFP at MOI 500. After stimulation with tumor necrosis factor-alpha (TNFα) or interleukin-1 (IL-1), luciferase activity was demonstrated in MSCs transduced with control virus, confirming upregulation of the NF-κB pathway. Conversely, MSCs transduced with AdsrIκB showed significantly less luciferase activity, indicating successful inhibition of the pathway. The NF-κB pathway was not stimulated by IL-6 in either group (B). Cytokines were used at a concentration of 100 ng/mL. Data are presented as mean ± standard error of the mean of triplicate experiments. *P <0.05 (two-way analysis of variance).

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