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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: TNFα and IL-1β influence the differentiation and migration of murine MSCs independently of the NF-κB pathway

Figure 2

Effect of inflammatory cytokines on osteogenesis of mouse mesenchymal stem cells (MSCs) in vitro . Osteogenesis was suppressed in a dose-dependent manner by both tumor necrosis factor-alpha (TNFα) and interleukin-1-beta (IL-1β). This was statistically significant in (A) BALB/c and (B) FVB MSCs compared with MSCs differentiated in the absence of pro-inflammatory cytokines. MSCs cultured in complete expansion medium only did not undergo differentiation (negative control). The dose-dependent inhibition of osteogenesis in FVB and BALB/cMSCs was demonstrated by alizarin red staining (C), and a representative micrograph of alizarin red staining of BALB/c MSCs is also shown (D). FVB MSCs stimulated to undergo osteogenesis and stained with alizarin red and control FVB MSCs are shown (E). Data shown are mean calcium per well ± standard error of the mean of triplicate experiments. *P <0.05, **P <0.001 (two-way analysis of variance).

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Stem Cell Research & Therapy

ISSN: 1757-6512