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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Novel daidzein analogs enhance osteogenic activity of bone marrow-derived mesenchymal stem cells and adipose-derived stromal/stem cells through estrogen receptor dependent and independent mechanisms

Figure 2

Effect of daidzein analogs on osteogenic differentiation of bone marrow-derived mesenchymal stem cells and adipose-derived stromal/stem cells is dose dependent. (A) Bone marrow-derived mesenchymal stem cells (BMSCs; n = 6) and (B) adipose-derived stromal/stem cells (ASCs; n = 6) were cultured in charcoal dextran-stripped osteogenic differentiation medium and simultaneously treated with vehicle, 17β-estradiol (E2), daidzein, or daidzein analog 2g or 2l at concentrations between 100 pm and 1 mM. After 14 days, cells were fixed, stained with alizarin red, destained with cetylpyridinium chloride, and measured at 590 nm. Osteogenic differentiation was determined relative to vehicle-treated cells normalized to 1.0. Bars, ± standard deviation.

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