Figure 6

Immunolocalization of PDX-1 in pancreas sections post pancreatectomy. (A) Few of the cells that extravasate from the blood vessels express PDX-1. (B), (C) These cells appear to migrate in large numbers towards the coelomic epithelium. PDX-1-positive cells are of varying shapes and size including few spherical cells with nuclear PDX-1. Varying size reflects migration of cells and also different stages of differentiation. (D) PDX-1-positive progenitor cells are observed along the coelomic epithelium. These cells differentiate into acinar cells along the coelomic epithelium. As these cells differentiate PDX-1, expression shifts from nuclear to cytoplasmic compartment and also staining intensity decreases. This suggests that PDX-1 protein that was initially required as a nuclear transcription factor eventually shifts to the cytoplasm and will eventually get degraded. (E) A newly assembled islet also shows faint positive staining for PDX-1. (F) Negative control. Scale bar represents 20 μm.