Figure 1From: Preconditioning of bone marrow mesenchymal stem cells by prolyl hydroxylase inhibition enhances cell survival and angiogenesis in vitro and after transplantation into the ischemic heart of ratsEffect of dedimethyloxalylglycine preconditioning on the expression of prosurvival and angiogenic factors. (A) Rat bone marrow mesenchymal stem cells (BMSCs) were cultured with or without dedimethyloxalylglycine (DMOG, 1Â mM) for 24Â hours. Hypoxia-inducible factor (HIF)-1É‘, glucose transporter 1 (Glut-1), vascular endothelial growth factor (VEGF) and phospho-Akt were then detected by western blotting. Beta-actin was used as the loading control. (B) Densitometric analysis was applied for comparison of the relative expression levels of different proteins in DMOG-BMSCs with respect to vehicle control BMSCs (C-BMSCs) that is arbitrarily presented as 1. n =3 independent assays. *P <0.05 compared with C-BMSC group. (C) Ratio of phospho-Akt against total Akt expression, quantified from the western blot gels. A large increase of 10-fold Akt phosphorylation was seen in DMOG-BMSCs compared with control BMSCs. n =3 independent assays. *P <0.05 vs. control cells.Back to article page