Figure 5

The ratio of Tregs and Th17 cells may contribute to SHED mediated treatment in MRL/ lpr mice. (A-C) Flow cytometric analysis showed that the number of CD25⁺Foxp3⁺ Tregs in CD4⁺ T lymphocytes of MRL/lpr spleen was not significantly changed in SHED and BMMSC transplantation (A). In contrast, SHED and BMMSC transplantation were capable of significantly reduced levels of CD4⁺IL17⁺ cells in spleen as compared to un-treated MRL/lpr mice (B). SHED transplantation significantly increased the ratio of Tregs and Th17 cells when compared to BMMSC transplantation group (C) (^[[[P < 0.001, ^[[P < 0.01, ^[P < 0.05). Results were shown as mean ± SD from un-treated, SHED and BMMSC MRL/lpr (n = 6). (D-F) Although SHED and BMMSC transplantations failed to alter IL10 (D) and IL6 (E) levels in serum of MRL/lpr mice, IL17 levels were significantly down-regulated in SHED and BMMSC transplanted group compared to un-treated MRL/lpr mice (F). Results were shown as means ± SD from un-treated, SHED and BMMSC MRL/lpr (n = 6).