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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Gene expression-based enrichment of live cells from adipose tissue produces subpopulations with improved osteogenic potential

Figure 1

Alkaline phosphatase liver/bone/kidney-based sorting method. Gene expression-based sorting of stromal vascular fraction (SVF) cells involved a 4-day osteogenic priming period prior to sorting. Next, cells were treated by electroporation with molecular beacons targeting alkaline phosphatase liver/bone/kidney (ALPL) mRNA. The beacon-treated cells were sorted using fluorescence-activated cell sorting (FACS) into ALPL+ and ALPL– populations. A subset of the initial, primed, SVF cells was left unsorted. The ALPL+, ALPL–, and unsorted cells were seeded onto tissue culture plates, differentiated down the osteogenic lineage, and assessed for production of calcified matrix.

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