Figure 2

Alkaline phosphatase liver/bone/kidney sort yields. Stromal vascular fraction cells treated with alkaline phosphatase liver/bone/kidney (ALPL) molecular beacon were sorted based on gene expression signals. (A) Cells were first gated using forward and side scatter measurements to eliminate debris and cellular aggregates. (B) Nonprimed cells were largely ALPL–, displaying a unimodal distribution overlapping with the primed ALPL– peak. (C) Sorting was determined using the bimodal distribution of primed SVF cells, with clear peaks existing for positive expressers (ALPL+) and negative expressers (ALPL–). A representative set of data is shown here for a single sorting experiment. 6-FAM, 6-carboxyfluorescein.