Figure 5

Multilineage differentiation of alkaline phosphatase liver/bone/kidney-sorted stromal vascular fraction cells. Primed, sorted stromal vascular fraction subpopulations were differentiated down the adipogenic and chondrogenic lineages to determine multipotency. ALPL+ samples showed significant increases in metabolite production over ALPL– and unsorted samples for both (A) adipogenic and (B) chondrogenic conditions. Of particular note, only ALPL+ samples showed a significant differentiation response for adipogenesis. ALPL– samples showed no response for either lineage, and unsorted samples only differentiated for chondrogenesis. Two-way analysis of variance with Holm–Sidak post hoc tests determined significance (*or nonmatching letters, P <0.05). ALPL, alkaline phosphatase liver/bone/kidney; sGAG, sulfated glycosaminoglycan.