Figure 3

Measurement of lactate dehydrogenase release and cell metabolism . Cells were seeded into 96-well plates and incubated for the indicated time points. The respective substrates were either added to the cells (cell metabolism) or to the cell culture supernatants (lactate dehydrogenase (LDH)). After the appropriate time and conditions, fluorescence was measured and given in arbitrary units. (A), (B) Cell metabolism on day 1 and day 3, respectively. (C), (D) LDH release on day 1 and day 3, respectively. Nontransfected cells (columns 5 and 6) were used as the respective controls. Significant differences observed between the Ambystoma mexicanum epidermal lipoxygenase (AmbLOXe)-expressing cells and the other cell populations are indicated (***P >0.001). Group 1, pIRES-EGFP-AmbLOXe (pI-AmbLOXe); Group 2, pmaxGFP^®; Group 3, 50% pI-AmbLOXe and 50% pmaxGFP^®; Group 4, cells nucleofected without DNA; Group 5, untreated adipose-derived stroma cells (ASCs); Group 6, untreated ASCs in lysis buffer.