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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Curcumin inhibits breast cancer stem cell migration by amplifying the E-cadherin/β-catenin negative feedback loop

Figure 3

Breast cancer stem cell (CSC)-enriched mammospheres exhibit highly aggravated migratory properties. (A, B) Representative phase-contrast images of cell adhesion and spreading assays of MCF-7 and 2° mammosphere-forming cells (left panels). The right panels demonstrate relative quantification of the data. (C) Confocal images showing focal adhesions in MCF-7 and 2° mammosphere-forming cells, stained with phospho-FAK (PE) (red) and nuclear stain 4′-6-diamidino-2-phenylindole (DAPI) (left panel). The right panel illustrates relative quantification data of mean focal adhesion area. (D) Representative brightfield images of transwell migration assays of MCF-7 and T47D cells and their respective 2° mammosphere-forming cells (left and middle panels). The right panel demonstrates relative quantification of the data graphically. (E) The percentage content of breast CSCs (CD44+/CD24-/low) in the migrated fractions of 2° mammospheres of MCF-7 and T47D cell lines as compared with non-stem cancer cells (NSCCs) was determined by flow cytometry and represented graphically (right panel). The left panel depicts representative flow cytometry data. Data are presented as mean ± standard error of mean or representative of three independent experiments.

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