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Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Curcumin inhibits breast cancer stem cell migration by amplifying the E-cadherin/β-catenin negative feedback loop

Figure 6

Curcumin abrogates breast cancer stem cell migration by restoration of E-cadherin expression. (A) Graphical illustration of transwell migration assay performed to study the effects of 24-hour curcumin treatment on migration of 2° mammosphere-forming cells of MCF-7 and T47D cell lines in a dose-dependent manner (right panels). Representative brightfield images are provided in the left panels. (B-D) Representative phase-contrast images of cell adhesion, spreading, and three-dimensional (3D) invasion assays of 2° mammospheres of MCF-7 cell line with or without curcumin treatment (left panels) along with their graphical quantifications (right panels). (E) Protein and mRNA expression profiles of E-cadherin in 2° mammospheres of MCF-7 cell line with or without curcumin treatment were determined by Western-blotting (left panel) and reverse transcription-polymerase chain reaction (RT-PCR) (right panel). (F) Graphical representation of relative mean fluorescence intensities (MFIs) in arbitrary units (AU) of epithelial markers cytokeratins 18 and 19 in 2° mammospheres of MCF-7 cell line with or without curcumin treatment were determined by flow cytometry and represented graphically (right panel). The left panel depicts representative flow cytometric histogram overlay data. (G) Graphical quantifications of cell adhesion, spreading, 3D invasion, and transwell migration assays of MCF-7-derived 2° mammospheres with or without curcumin and E-cadherin-short hairpin RNA (shRNA) treatment/transfection. (H) Transwell migration assay was performed under similar experimental conditions as mentioned above in T47D-derived 2° mammospheres. α-Actin/glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal loading control. Data are presented as mean ± standard error of mean or representative of three independent experiments. Cont, control; Cur, curcumin.

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