Figure 2
From: Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes
Representative images of hPSC and hPSC-CM. (A) Representative images of human pluripotent stem cells (hPSCs) (left), a monolayer culture of hPSC-derived cardiomyocytes (hPSC-CMs; unstained, middle), and dissociated and re-plated human embryonic stem cell-derived cardiomyocytes immunostained with antibodies against cardiac troponin T (TNNT2; right) [139]. (B) Cardiac troponin I (TNNI3) immunostaining of a monolayer culture of human induced pluripotent stem cell-derived cardiomyocytes at day 29 of differentiation showing random patterns of striations. (C) Immunostaining of a three-dimensional tissue strip with well-aligned troponin-stained hPSC-CMs. Green, TNNT2 labeling (A, C), TNNI3 labeling (B); blue, DAPI labeling.