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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes

Figure 3

Optical characterization of hPSC-CM electrophysiology. Optical mapping of enzymatically digested and re-plated human induced pluripotent stem cell-derived cardiomyocyte monolayers recorded 9 days (A-C) and 13 days (D-F) after re-plating. (A) Transmembrane voltage map of 9-day re-plated monolayer. (B) Activation time map and local conduction velocity vectors of (A). (C) Action potential recorded from location indicated by white box in (A). Electrical coupling among the cells of this 9-day old monolayer is poor, as indicated by the disorganized activation time map, rough wavefront of the propagating AP and slow conduction velocity (5.4 cm/second). (D) Transmembrane voltage map of a 13-day re-plated monolayer. (E) Activation time map and local conduction velocity vectors of (D). (F) Action potential recorded from location indicated by white box in (D). Electrical coupling was much improved with increased time in culture, as indicated by the near planar propagating action potential as well as faster conduction velocity (10.5 cm/second). Dashed lines in (C) and (F) indicate the time points of the corresponding transmembrane voltage maps in (A) and (D).

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