VEGF165 gene-modified rat hair follicle stem cells. (a) Fluorescein isothiocyanate image using fluorescence microscopy. Green staining indicates that the target plasmid pLV–VEGF165–IRES–EGFP was successfully transfected into the hair follicle stem cells (HFSCs). (b) Phase contrast image. (c) Reverse transcription-polymerase chain reaction for VEGF165 expression after transfection. (d) Western blot for expression of VEGF165 protein. Scale bars: 100 μm (a, b). EGFP, enhanced green fluorescent protein; IRES, internal ribosome entry site; VEGF, vascular endothelial growth factor.