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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Fibroblasts derived from human embryonic stem cells direct development and repair of 3D human skin equivalents

Figure 1

Characterization of hES-derived mesenchymal cell lines. (a) Schematic summarizing the two protocols used for generating EDK and H9-MSC from H9 human embryonic stem (hES) cells. (b) Cellular morphology of human foreskin fibroblasts (HFF), EDK, and H9-MSC using phase contrast. Bars, 50 μm. (c) Expression of mesenchymal markers vimentin, Thy1, and α-SMA following immunofluorescence staining. Bars, 50 μm. (d) Comparison of surface antigen expression on HFF, EDK, and H9-MSC by flow cytometric analysis. EDK and H9-MSCs express markers associated with mesenchymal phenotype (positive for expression CD73, CD105, CD44, CD13, CD106, and CD166; negative for expression of CD45, CD34, and CD31). EDK and H9-MSCs show significant differences in expression of CD146 and CD10 antigens. (HFF, filled grey profile; EDK, red profile; H9-MSC, black profile).

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