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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Fibroblasts derived from human embryonic stem cells direct development and repair of 3D human skin equivalents

Figure 3

EDK cells but not H9-MSC promote epidermal morphogenesis in HSE tissues. (a): Tissue morphology of human skin-equivalent (HSE) tissues constructed using human foreskin fibroblasts (HFF), EDK, H9-MSC, or without cells as analyzed by H&E staining. (b) The expression pattern of the epidermal differentiation marker keratin 1/10 was analyzed by immunofluorescence (red). Bars, 50 μm. (c) Comparison of the secretory profile of EDK, H9-MSC, and HFF cells by antibody-based cytokine array (d) Histogram profiles generated by quantifying the mean spot pixel densities by ImageJ from the array membrane shown above. The data are presented as percentages of the respective positive controls. (e) Hepatocyte growth factor (HGF) mRNA expression in HFF, EDK, and H9-MSC by real-time RT-PCR. Data are normalized to GAPDH, and all results are expressed as the mean +/- standard deviation (SD) of three experiments and three technical replicates per experiment. (f) Levels of HGF in supernatants from HFF, EDK, and H9-MSC monolayer cultures by ELISA. Data are normalized per 104 cells and all results are expressed as the mean +/- SD of three experiments and three technical replicates per experiment.

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