Mouse model of lung injury with bleomycin. (A) Schematic diagram of the experimental plan for inducing peribronchial fibrosis. (B) Gross anatomy representative of normal and fibrotic mouse lungs used in the study. Right lungs of Nestin-GFP/NG2-DsRed mice were used for classical histology (C), and left lungs for immunohistochemistry (Figure 2A,B). (C) Representative sections of normal mouse lungs and fibrotic lungs collected 14 days after intratracheal administration of bleomycin. Images of sections stained with hematoxylin and eosin (H&E) for general morphology and van Gieson (pink) and Masson’s trichrome (blue) for collagen deposition throughout the lungs.