Mouse model of unilateral ureteral obstruction. (A) Schematic diagram showing unilateral ureteral obstruction (UUO) in Nestin-GFP/NG2-DsRed transgenic mice. The right ureter was exposed via a lateral incision and ligated. The right obstructed kidney or left nonobstructed kidney (control) was analyzed 14 days after the operation. (B) Gross anatomy representative of normal and fibrotic mouse kidneys used in the study. (C) Histology of UUO and contralateral kidneys. Paraffin kidney sections were stained with hematoxylin and eosin (H&E). Collagen content was assessed by van Gieson (pink) and Masson’s trichrome (blue).