Figure 4From: Human liver stem cells and derived extracellular vesicles improve recovery in a murine model of acute kidney injuryEffect of concentrated CM depleted of EVs on AKI recovery. Creatinine (A) and BUN (B) were measured in AKI mice treated with vehicle alone or with CM depleted (AKI + CM w/o EVs) or not of EVs (AKI + CM + EVs) five days after glycerol injection. Data are expressed as mean ± SD; Analysis of variance with Newmann-Keuls multicomparison test was performed; *P <0.05 CM-treated AKI mice versus vehicle-treated AKI mice; #P <0.05 CM + EVs- treated AKI mice versus CM w/o EVs-treated AKI mice. (C) Comparison of tubular morphology in AKI mice injected with HLSC-CM depleted or not of EVs. Data are expressed as mean ± SD of hyaline cast and tubular necrosis observed under high power (original magnification: ×400). Analysis of variance with Newmann-Keuls multicomparison test *P <0.05 CM = treated AKI mice versus vehicle-treated AKI mice, #P <0.05 CM + EV-treated AKI mice versus CM w/o EV-treated AKI mice. Quantification of PCNA (D) and BrdU (E) positive cells/hpf in AKI mice treated with vehicle alone or CM with or without (w/o) EVs and CM + EVs and sacrificed after five days. BrdU was injected intraperitoneally for two successive days before mice were killed. Data are expressed as mean ± SD. ANOVA with Dunnet’s multicomparison test was performed: * P <0.05 CM + EV-treated AKI mice versus vehicle-treated AKI mice. AKI, acute kidney injury; ANOVA, analysis of variance; BrdU, 5-bromo-2′-deoxy-uridine; BUN, blood urea nitrogen; CM, conditioned medium; EV, extracellular vesicles; HLSCs, human liver stem cells; hpf, high power field; PCNA, proliferating cell nuclear antigen; SD, standard deviation; w/o, without.Back to article page