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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Human liver stem cells and derived extracellular vesicles improve recovery in a murine model of acute kidney injury

Figure 4

Effect of concentrated CM depleted of EVs on AKI recovery. Creatinine (A) and BUN (B) were measured in AKI mice treated with vehicle alone or with CM depleted (AKI + CM w/o EVs) or not of EVs (AKI + CM + EVs) five days after glycerol injection. Data are expressed as mean ± SD; Analysis of variance with Newmann-Keuls multicomparison test was performed; *P <0.05 CM-treated AKI mice versus vehicle-treated AKI mice; #P <0.05 CM + EVs- treated AKI mice versus CM w/o EVs-treated AKI mice. (C) Comparison of tubular morphology in AKI mice injected with HLSC-CM depleted or not of EVs. Data are expressed as mean ± SD of hyaline cast and tubular necrosis observed under high power (original magnification: ×400). Analysis of variance with Newmann-Keuls multicomparison test *P <0.05 CM = treated AKI mice versus vehicle-treated AKI mice, #P <0.05 CM + EV-treated AKI mice versus CM w/o EV-treated AKI mice. Quantification of PCNA (D) and BrdU (E) positive cells/hpf in AKI mice treated with vehicle alone or CM with or without (w/o) EVs and CM + EVs and sacrificed after five days. BrdU was injected intraperitoneally for two successive days before mice were killed. Data are expressed as mean ± SD. ANOVA with Dunnet’s multicomparison test was performed: * P <0.05 CM + EV-treated AKI mice versus vehicle-treated AKI mice. AKI, acute kidney injury; ANOVA, analysis of variance; BrdU, 5-bromo-2′-deoxy-uridine; BUN, blood urea nitrogen; CM, conditioned medium; EV, extracellular vesicles; HLSCs, human liver stem cells; hpf, high power field; PCNA, proliferating cell nuclear antigen; SD, standard deviation; w/o, without.

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