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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: 17beta-estradiol promotes the odonto/osteogenic differentiation of stem cells from apical papilla via mitogen-activated protein kinase pathway

Figure 2

Effects of E2 on morphology and proliferation of SCAP. (A) MTT assay at day 5 for E2-treated SCAP at different concentrations (concentration screening). (B) ALP activity in E2-treated SCAP at different concentrations (concentration screening). (C) The morphology of SCAP in the control group. (D) A higher magnification of (C). (E) The morphology of SCAP in the E2 group. (F) A higher magnification of (E). (G) Flow cytometry analysis of control SCAP. (H) Flow cytometry analysis of E2-treated SCAP. (I) Average proliferation indexes (PI = S + G2M) in the control group (6.67%) and the E2 group (7.37%, P >0.05). (J) Growth curves for control and E2-treated SCAP. (K) Colony forming assay for control and E2-treated SCAP. (L) Average number of colonies formed in the control group (14) and the E2 group (16.5, P >0.05). Values are given as the mean ± SD, n = 3. *P <0.05, **P <0.01. ALP, alkaline phosphatase; E2, 17beta-estradiol; MTT, methyl-thiazolyl-tetrazolium; SCAP, stem cells from apical papilla; SD, standard deviation.

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Stem Cell Research & Therapy

ISSN: 1757-6512