Skip to main content
Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Human neural stem cells rapidly ameliorate symptomatic inflammation in early-stage ischemic-reperfusion cerebral injury

Figure 1

Human NSC transplantation ameliorates behavioral deficits. I. (A) Experimental timeline. MCAO/R was performed in C57BL/6 J mice at time 0, and hNSCs were transplanted into the ipsilesional hippocampus 24 hours later. Outcomes were assessed at the indicated intervals. Tx, transplantation. (B) Relative cerebral blood flow (rCBF) was measured by laser Doppler flowmetry over the area supplied by the MCA. Pre-ischemic rCBF was assigned a value of 100%. Subsequent values are presented as a percentage of that value. MCAO promoted an 80% decrease in rCBF (86.1 ± 1.6%), and the reperfusion flow rate 60 minutes post-MCAO was 90.1 ± 4.0% (n = 18). II. (A) In the forepaw adhesive removal tests, the mean adhesive removal time for hNSC-transplanted mice was significantly shorter than that of MCAO/R mice, showing improvement (n = 14). (B) In the beam walk tests, hNSC-transplanted MCAO/R mice improved neurological dysfunction as shown by crossing the beam in significantly less time than did nontransplanted MCAO/R mice (n = 14). (C) In the rotarod tests, hNSC-transplanted mice improved motor dysfunction as shown by remaining on the rod for a significantly longer time than did nontransplanted MCAO/R mice (n = 14). Four velocities (10, 15, 20, and 25 rpm) were used, and three trials were performed at each velocity. Durations at all speeds are summed, and data are presented as a percentage relative to the sham mice. **P <0.01, ***P <0.001 versus sham mice; ##P <0.01; ###P <0.001 versus nontransplanted MCAO/R mice. Data are expressed as mean ± SEM. MCA, middle cerebral artery; MCAO/R, middle cerebral artery occlusion with reperfusion; NSC, neural stem cells; SEM, standard error of the mean.

Back to article page