Skip to main content
Figure 1 | Stem Cell Research & Therapy

Figure 1

From: microRNA-378 promotes mesenchymal stem cell survival and vascularization under hypoxic–ischemic conditions in vitro

Figure 1

Mesenchymal stem cell proliferation after the transfection of microRNA-378. (A) miR-378 expression status after transfection with microRNA (miRNA) mimic detected by quantitative real-time polymerase chain reaction. (B) Mesenchymal stem cell (MSC) proliferation rate tested by MTS assay (cellTiter96AQ, one solution cell proliferation assay, catalogue number G3582; Promega, Madison, Wisconsin, USA). *P < 0.05; **P < 0.01. (C) Proliferation rate = optical density (OD) values at other time points divided by OD value at the beginning × 100% (same sample). (D) Inhibition rate = (1 – OD value divided by native MSC group OD value) × 100% (same time). Native MSCs, MSCs cultured in normoxic conditions; miR-378, miR-378-transfected MSCs cultured in normoxic conditions; MSCs + hypoxia, MSCs cultured in hypoxic conditions; miR-378 + hypoxia, miR-378-transfected MSCs cultured in hypoxic conditions.

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512