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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Human mesenchymal stem cells possess different biological characteristics but do not change their therapeutic potential when cultured in serum free medium

Figure 1

In vitro growth characteristics of human umbilical cord mesenchymal stem cells cultured in serum-free medium. Both in SCM (a) and in SFM (b), human umbilical cord mesenchymal stem cells (hUC-MSCs) maintained fibroblast-like morphology (40×). (c) Calculated population-doubling time. Open boxes, hUC-MSCs expanded in SFM; filled boxes, hUC-MSCs expanded in serum-containing medium (SCM). SFM-expanded hUC-MSCs possessed a much longer calculated population-doubling time. (d) In vitro lifespan of hUC-MSCs derived from five different donors. (e) Paired t test was used to compare the lifespan of hUC-MSCs cultured in SFM and SCM. A significant different lifespan between SFM-derived and SCM-derived hUC-MSCs was observed. (f) Senescence-associated β-galactosidase activity analysis of SFM-expanded hUC-MSCs at late passage. Blue stain shows senescent cells (×200).

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Stem Cell Research & Therapy

ISSN: 1757-6512