Figure 3

Array-based comparative genomic hybridization analysis of human umbilical cord mesenchymal stem cells expanded in serum-free medium and serum-containing medium. Huge unbalanced genome alteration was not obvious in the single-panel rainbow plot, in which each chromosome was differentiated by color. (a) Sample 2 in serum-free medium (SFM). (b) Sample 2 in serum-containing medium (SCM). (c) Sample 4 in SFM. (d) Sample 4 in SCM. (e) A copy number variation (CNV) segment (chr3:181315609 to 181344028), which was observed in both of the SFM-expanded human umbilical cord mesenchymal stem cells (hUC-MSCs; red arrow), was not observed in either of the SCM-expanded samples (blue arrow). (f) CNV identified by array-based comparative genomic hybridization in culture. Amplifications and deletions were mapped onto the human genome for four hUC-MSC clones. Each individual CNV is marked: red circle, amplification; green circle, deletion.