Figure 1

Adipose-derived mesenchymal stem cells remain diploid under various culture conditions. Adipose-derived mesenchymal stem cells (ASCs) were characterized according to (A) their differentiation potential and (B) their surface marker expression (percentage reflects cell-staining above background). (C) Five adipose-derived mesenchymal stem cell preparations were grown in different growth media (as indicated: two preparations in Mesencult medium, two preparations in ADSC medium and one preparation in Dulbecco’s modified Eagle’s medium (DMEM)) and their DNA content was analyzed by flow cytometry at early and late passages, diploid (2N) and tetraploid (4N). Primary diploid spleen cells used as control.