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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Relative genomic stability of adipose tissue derived mesenchymal stem cells: analysis of ploidy, H19 long non-coding RNA and p53 activity

Figure 3

Independent adipose-derived mesenchymal stem cell preparations retain their diploid state under hypoxia and react homogeneously to changing oxygen conditions. Independent preparations of paired (A) adipose-derived mesenchymal stem cells (ASCs) and (B) bone marrow mesenchymal stem cells (BM MSCs; prepared from the same male or female mouse) were expanded in Mesencult under hypoxic conditions (3% oxygen) and their DNA content was analyzed by flow cytometry. Resulting plots from male/female adipose-derived mesenchymal stem cells/BM MSCs were overlaid and are presented in a single graph, diploid (2N) and tetraploid (4N); six female and six male adipose-derived mesenchymal stem cells and six total female and male BM MSCs were used. (C) Graph summarizing the percentage of adipose-derived mesenchymal stem cells and BM MSCs that became polyploid out of all cell preparations cultured during the study. (D), (E) (F) Comparison of the RNA level of independently derived adipose-derived mesenchymal stem cells or BM MSCs that were expanded either in hypoxic (H) or normoxic (N; normal oxygen) conditions made by quantitative real-time PCR. *P <0.05, Mann–Whitney test (each group size is mentioned in Table 1). HIF1α, hypoxia-inducible factor 1α; VEGFa, vascular endothelial growth factor A.

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