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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia

Figure 5

Enhanced MSC survival by lentiviral vector modification in hypoxia. (A) Percentage rMSC viability as determined by MTT assay after hypoxia exposure (*p < 0.05 HSP70-MSCs and SOD1-MSCs vs. non-transduced MSCs). (B) DAPI staining of MSCs after 72 h hypoxia. (C) Percentage apoptotic nuclei per microscope field (n = 10) at 72 h hypoxia (**p < 0.001 HSP70 MSCs vs. non-transduced MSCs). (D) Caspase-3 activity in MSC after 48 h and 72 h hypoxia (*p < 0.05 HSP70-MSCs vs. non-transduced MSC). Data are shown as mean ±SD (n = 3) or as representative images of three independent experiments.

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