Figure 6

Enhanced MSC survival by lentiviral vector modification in ischaemia. (A) Percentage rMSC viability as determined by MTT assay after ischaemia exposure (*p < 0.05 HSP70-MSCs vs. non-transduced MSC). (B) DAPI staining of MSCs after 72 h ischaemia. (C) Percentage apoptotic nuclei per microscope field (n = 10) (**p < 0.001 HSP70-MSCs vs. non-transduced MSCs at 48 h and 72 h ischaemia). (D) Caspase-3 activity in rMSC after 48 h and 72 h ischaemia (*p < 0.05 HSP70-MSCs vs. non-transduced MSCs). Data are shown as mean ±SD (n = 3) or as representative images of three independent experiments.