Figure 7

Enhanced MSC survival by lentiviral vector modification in ischaemia with glycolysis inhibition - complete glucose deprivation. (A) Percentage rMSC viability as determined by MTT assay after ischaemia with 2DG exposure (*p < 0.05 HSP70-MSC vs. non-transduced MSC). (B) DAPI staining of MSCs after 72 h ischaemia + 2DG. (C) Percentage apoptotic nuclei per microscope field (n = 10) (*p < 0.05, **p < 0.001 HSP70-MSCs vs. non-transduced MSC). (D) Caspase-3 activity in MSC after 48 h and 72 h ischaemia + 2DG (*p < 0.05 HSP70-MSCs vs. non-transduced MSC). Data are shown as mean ±SD (n = 3) or as representative images of three independent experiments.