Figure 8

Enhanced MSC differentiation by lentiviral vector modification in hypoxia and ischaemia. (A) Oil Red O staining for lipid vacuoles post rMSC adipogenic differentiation. There was no negative effect of lentivirus vector transduction and over-expression of GFP or HSP70 on MSC adipogenic differentiation capacity. (B) Numbers of lipid-containing cells per microscope field (n = 10) (**p < 0.001 HSP70-MSCs vs. non-transduced MSC). (C) Oil Red O quantity (*p < 0.05, **p < 0.001 HSP70-MSCs vs. non-transduced MSC). Data are shown as mean ±SD (n = 3) or as representative images of three independent experiments.