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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Human mesenchymal stem cells/multipotent stromal cells consume accumulated autophagosomes early in differentiation

Figure 5

Immortalized human mesenchymal stem cells show little autophagosome turnover unless stimulated. Immortalized human mesenchymal stem cells grown under normal culture conditions were tagged with a tandem green fluorescent protein-red fluorescent protein (GFP-RFP) light chain 3 (LC3) via viral transfection (see Materials and methods). ihMSCs were then stimulated with bafilomycin to halt autophagy for 1 hour or with rapamycin to induce autophagy for 15 minutes and 1 hour. The representative of experiments repeated at least three times is shown.

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