Figure 5From: Human mesenchymal stem cells/multipotent stromal cells consume accumulated autophagosomes early in differentiationImmortalized human mesenchymal stem cells show little autophagosome turnover unless stimulated. Immortalized human mesenchymal stem cells grown under normal culture conditions were tagged with a tandem green fluorescent protein-red fluorescent protein (GFP-RFP) light chain 3 (LC3) via viral transfection (see Materials and methods). ihMSCs were then stimulated with bafilomycin to halt autophagy for 1Â hour or with rapamycin to induce autophagy for 15Â minutes and 1Â hour. The representative of experiments repeated at least three times is shown.Back to article page