Figure 2From: Leukocyte transmigration into tissue-engineered constructs is influenced by endothelial cells through Toll-like receptor signalingImplantation of tissue-engineered constructs and subsequent cell migration. Experimental design of the in vivo study is presented in (A), and a macroscopic image of the scaffold used is shown in (B). Stained samples (hematoxylin and eosin) at 1 and 3 weeks (C) are shown at 4×, 20×, and 60× magnification with scale bars of 200, 50, and 10 μm, respectively. Multinucleated giant cells on the scaffold surface (S) can be seen at high magnification. (D) Quantification of cells migrated into the construct during the experimental period (***P <0.001, n = 6). (E) Nuclei of migrated cells visualized with 4′,6-diamidino-2-phenylindole (DAPI) staining. EC, endothelial cell; MSC, multipotent stromal cell.Back to article page