Equilibrium partitioning by ionic contrast-agent microcomputed tomography (EPIC-microCT). Experimental osteoarthritis was induced by intra-synovial injection of monosodium iodoacetate (MIA) and evaluated at week 3 by EPIC-microCT. Excised rat femurs were equilibrated with an ionic contrast agent (hexabrix) and scanned to assess cartilage and subchondral bone. The arthritic group exhibited substantial sulfated glycosaminoglycan depletion, cartilage degradation, and subchondral bone resorption, illustrating the capacity of contrast-enhanced microCT to quantitatively assess cartilage and bone in preclinical models of osteoarthritis. Arrows indicate location of complete cartilage degradation in MIA group and corresponding location in the control. L, lateral; M, medial. Figure reproduced with permission from John Wiley & Sons, Inc. .