Neural markers were differentially expressed between
-OFF human embryonic stem cell (hESC)-differentiated cells during neural lineage differentiation. (A) Neural lineage differentiation was conducted from the undifferentiated stage to the 18 day-Matrigel attachment stage in NTU1 and NTU3 hESC lines. (B) Expression levels of stage-specific markers were analyzed by quantitative reverse transcription-polymerase chain reaction in MEG3-ON and MEG3-OFF groups during differentiation. The quantitation of mRNA expression was performed by using the 2−ΔΔCp method (using the housekeeping gene GAPDH for normalization). Error bars represent the standard error of the mean generated from three biological samples with three technical repeats each. *P <0.05, **P <0.01 compared with the respective MEG3-ON groups by Student’s t test. MEG3, maternally expressed gene 3; NES, neuroectodermal sphere.