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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Loss of non-coding RNA expression from the DLK1-DIO3 imprinted locus correlates with reduced neural differentiation potential in human embryonic stem cell lines

Figure 5

Neural markers were differentially expressed between MEG3 -ON and MEG3 -OFF human embryonic stem cell (hESC)-differentiated cells during neural lineage differentiation. (A) Neural lineage differentiation was conducted from the undifferentiated stage to the 18 day-Matrigel attachment stage in NTU1 and NTU3 hESC lines. (B) Expression levels of stage-specific markers were analyzed by quantitative reverse transcription-polymerase chain reaction in MEG3-ON and MEG3-OFF groups during differentiation. The quantitation of mRNA expression was performed by using the 2−ΔΔCp method (using the housekeeping gene GAPDH for normalization). Error bars represent the standard error of the mean generated from three biological samples with three technical repeats each. *P <0.05, **P <0.01 compared with the respective MEG3-ON groups by Student’s t test. MEG3, maternally expressed gene 3; NES, neuroectodermal sphere.

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