Neurite formation was reduced in
-OFF human embryonic stem cell (hESC)-differentiated cells compared with cells differentiated from
-ON hESCs. Immunofluorescent staining was performed with anti-beta-III Tubulin and anti-MAP2 antibodies to examine neurite formation in cells derived from MEG3-ON and MEG3-OFF hESCs of the NTU1 (A) and NTU3 (B) cell lines after 18 days of differentiation on Matrigel. Scale bars, 100 μm. MEG3, maternally expressed gene 3.