Isolation of primitive cells from lipoaspirates and lipoaspirates enriched with stem cells. Histograms represent (A) cell yield, (B) cell composition and (C) number of colony-forming units at 7 days from seeding. (D, E, F, G) Expression of stem cell markers in primary culture. Proliferating cells expressed high levels of Nanog (green fluorescence, D), Sox-2 (yellow fluorescence, E) and Oct-4 (magenta fluorescence, F), while a fraction of cells expressed c-Kit (red fluorescence, G). Nuclei are depicted by the blue fluorescence of 4′,6-diamidino-2-phenylindole. In the histograms, results are expressed as mean ± standard deviation. For each isolation method, *P <0.05 vs. lipoaspirates. Cytori, Cytori Celution System (Cytori Ltd., Deeside, UK) ; Fastem, Fastem Corios (CORIOS Soc. Coop, San Giuliano Milanese (MI), Italy); L, lipoaspirates; L + SC, stem cell-enriched lipoaspirates; Lipokit, Lipokit Medikhan System (Medikan International Inc, Pusan, Korea) .